HCID 15932587, 화합물 → HCID 54227580, 화합물

HRID 1656866노트

반응식

HRID 1656866 반응식
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([CH2:32][O:33][C@@H:34]2[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([OH:39])[C@H:36]([OH:37])[C@H:35]2[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:19]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([CH2:32][O:33][C@@H:34]4[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([O:39][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:36]([OH:37])[C@H:35]4[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:30][C@H:31]([CH2:32][OH:33])[C@H:16]([OH:15])[C@H:17]([OH:49])[C@H:18]1[OH:48]

참가 물질

5

반응물 2

HCID 15932587HCID 15932587, 화합물HCID 54584734HCID 54584734, 화합물

생성물 1

HCID 54227580HCID 54227580, 화합물

시약·촉매·용매 2

HCID 10313134Manganese(2+) dichloride촉매HCID 2724247Sodium Cacodylate용매

실험 절차

A solution of compound 20 of Example 20 (72 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 98 mg. The structural identity of titled compound 26 was unambiguously assigned by 1H-- and 13C-n.m.r (see Tables 4 and 5, respectively).

후처리

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

출처 및 근거

ORD 기록
ord-58e7e751f49346a7a40069fc1370da35
출처 데이터셋
uspto-grants-1993_10
특허 번호
US05254676

반응 노트

불러오는 중…