Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Glycerol → Bistris
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Glycerol | HCID 753 | — |
| 반응물 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 반응물 | Bromophenol Blue | HCID 8272 | — |
| 반응물 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 반응물 | Sodium Lauryl Sulfate | HCID 3423265 | — |
| 생성물 | Bistris | HCID 81462 | 10% |
반응 단계
Protein samples were prepared for SDS-PAGE by diluting 1:1 with 2× denaturing buffer (40 μg/ml SDS, 20% glycerol, 30 μg/ml DTT and 10 μg/ml bromophenol blue in 125 mM Tris, pH 6.8), and boiling for 2 min. Pre-cast Nu-PAGE 10% Bis-Tris protein gels were obtained from Invitrogen (Carlsbad, Calif.). Each lane was loaded with a maximum of 10 μl of sample, and run for 50 min at 200V. After electrophoresis was complete, the gel was stained with Invitrogen SafeStain (1 hour in staining solution, then washed overnight with water).
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US08000904B2 |
| 출처 데이터셋 | uspto-grants-2011_08 |
| ORD ID | ord-3f04a97701c145e6bd734766b4a10cec |
| 출처 메모 | uspto-grants-2011_08 |