Benzoylarginine ethyl ester → Citrulline

HRID 1676880

반응식

HRID 1676880 반응식
CN(C1C2C[C@@H]3C(C(=O)C=2C(O)=CC=1)=C(O)[C@@]1(O)[C@H]([C@H](N(C)C)C(O)=C(C(N)=O)C1=[O:18])C3)C.C[C@]1(O)[C@@H]2C(=C(O)[C@]3(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=C(Cl)C1=2.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.CC[O:109][C:110]([C@@H:112]([NH:120]C(C1C=CC=CC=1)=O)[CH2:113][CH2:114][CH2:115][N:116]=[C:117](N)[NH2:118])=[O:111]>>[NH2:120][C@H:112]([C:110]([OH:109])=[O:111])[CH2:113][CH2:114][CH2:115][NH:116][C:117]([NH2:118])=[O:18]

참가 물질

5

반응물 4

HCID 123880Benzoylarginine ethyl esterHCID 178482881HCID 178482881, 화합물HCID 178484163HCID 178484163, 화합물HCID 178484872HCID 178484872, 화합물

생성물 1

HCID 9750Citrulline

실험 절차

Inhibition constants were determined for minocycline, chlortetracycline, and streptomycin using standard kinetic analyses. Briefly, the compounds were incubated in Reaction Buffer containing various concentrations of BAEE (0-20 mM) for 10 minutes at 37° C. PAD4 (0.2 μM) was then added to initiate the reaction and allowed to proceed for 6 additional minutes. Reactions were then quenched by flash freezing in liquid N2 and the amount of Cit produced was quantified. The data obtained for streptomycin, minocycline and chlortetracycline were fit to equations 2 and 3,

후처리

  1. additionPAD4 (0.2 μM) was then added
  2. customthe reaction
  3. customReactions were then quenched by flash

출처 및 근거

ORD 기록
ord-3a2d3b4530144744a84496a8d9c05e64
출처 데이터셋
uspto-grants-2011_08
특허 번호
US08007996B2

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