Bicine → Acetate

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The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing lithium L-lactate (0.750M), FMN (0.01 mM), isobutyfic acid (HPLC internal standard, 0.100M), bicine buffer (0.788M), soluble spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 8.9 (adjusted with 50% NaOH) and the reaction run at 5° C. After 28.5 hours, the HPLC yields of pyruvate and acetate were 47.7% and 43.6%, respectively, and 11.5% lactate remained. The remaining activity of glycolate oxidase and catalase were 40% and 100%, respectively, of their initial values.

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