반응식
C(=O)=[O:2].C[C@@H](NC)[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)CC1.C[C@@H]1[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@@]2(O)O[C@H:55]([C@H:56]([C:88]([OH:90])=[O:89])[C@@H](O)C2)[CH2:54][C@@H:53](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](N)[C@@H]2O)[CH:52]=[CH:51][CH:50]=[CH:49][CH:48]=[CH:47][CH:46]=[CH:45][CH:44]=[CH:43][CH:42]=[CH:41][CH:40]=C1>>[C:88]([O:90][OH:2])(=[O:89])[CH2:56][CH2:55][CH2:54][CH2:53][CH2:52][CH2:51][CH2:50]/[CH:49]=[CH:48]\[CH2:47]/[CH:46]=[CH:45]\[CH2:44][CH2:43][CH2:42][CH2:41][CH3:40]참가 물질
4
실험 절차
Human endothelial cells were cultivated at 37° C. in Leighton tubes and in an atmosphere of saturated humidity constituted by a gaseous mixture of 95% air and 5% of CO2. The culture medium was constituted by an EGM (Clonetics) medium, pH=7.4 containing 2% of foetal calf serum, 10 ng/ml of recombinant human growth factor EGF, 10 μg/ml of heparin, 50 μm/ml of gentamicin and 50 μg/ml of amphotericin B.
후처리
- customwere cultivated at 37° C. in Leighton tubes and in an atmosphere of saturated humidity
출처 및 근거
- ORD 기록
- ord-0cbc96ff4c69492ea902f6dc79e71cc7
- 출처 데이터셋
- uspto-grants-1999_10
- 특허 번호
- US05968920
연결된 노트
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