Phosphate → N-sulfonyl benzyloxycarbonylthreonine amide

A solution of 2-picoline (17.8 ml) in 90 ml of methylene chloride was cooled to -5° C. (ice-brine) and chlorosulfonic acid (5.97 ml) was added at such a rate as to maintain the internal reaction temperature below 5° C. The resulting solution was added via canula, to a suspension of 7.56 g of benzyloxycarbonylthreonine amide, O-mesylate in 120 ml of methylene chloride. The resulting heterogeneous mixture was refluxed for about 16 hours yielding a clear solution. The solution was poured into 500 ml of pH 4.5 phosphate buffer (0.5M) and further diluted with 120 ml of methylene chloride. The separated organic layer was washed once with 100 ml of buffer solution and the combined aqueous phases were treated with 10.2 g of tetra-n-butyl-ammonium hydrogensulfate and extracted with methylene chloride (one 300 ml portion and two 150 ml portions). After drying the combined organic extracts over sodium sulfate, the solution was concentrated to yield 12.7 g of N-sulfonyl benzyloxycarbonylthreonine amide, O-mesylate, tetrabutylammonium salt.

1. additionwas added at such a rate as
2. temperatureto maintain the internal reaction temperature below 5° C
3. temperatureThe resulting heterogeneous mixture was refluxed for about 16 hours
4. customyielding a clear solution
5. washThe separated organic layer was washed once with 100 ml of buffer solution
6. additionthe combined aqueous phases were treated with 10.2 g of tetra-n-butyl-ammonium hydrogensulfate
7. extractionextracted with methylene chloride (one 300 ml portion and two 150 ml portions)
8. dry with materialAfter drying the combined organic extracts over sodium sulfate
9. concentrationthe solution was concentrated

ORD 기록

ord-8342a2308778493ca15e47b303e03638

출처 데이터셋

uspto-grants-1989_05

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US04826973