Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Tris(hydroxymethyl)aminomethane → Phospho-L-tyrosine
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 반응물 | Fenticlor | HCID 7329 | — |
| 반응물 | Sodium Lauryl Sulfate | HCID 3423265 | — |
| 반응물 | Anti-phosphotyrosine | HCID 11196273 | — |
| 촉매 | Sodium orthovanadate | HCID 61671 | — |
| 생성물 | Phospho-L-tyrosine | HCID 30819 | — |
반응 단계
Cells were starved (24 hours for MCF-7, 4 hours for 32D derived cell lines) before addition of the indicated amounts of growth factors. Total cell lysates were prepared by addition of SDS PAGE sample buffer (1% SDS, 0.15M Tris pH 8.6, 5% BME and 1 mM Sodium Ortho Vanadate) directly to cells. Cell lysates and were run on 8–16% Tris-Glycine gradient gels (Novex). Proteins were transferred onto Hybond ECL nitrocellulose membranes (Amersham) and were immunoblotted with anti-phosphotyrosine MAb.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07049409B2 |
| 출처 데이터셋 | uspto-grants-2006_05 |
| ORD ID | ord-2a606c8a2cd546bfa6dd419a50819e9c |
| 출처 메모 | uspto-grants-2006_05 |