참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Phenylmethylsulfonyl Fluoride | HCID 4784 | — |
| 반응물 | 5,5'-Dithiobis(2-nitrobenzoic Acid) | HCID 6254 | — |
| 반응물 | Bicinchoninic acid | HCID 71068 | — |
| 반응물 | [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [3-hydroxy-2,2-dimethyl-4-oxo-4-[[3-oxo-3-(2-sulfanylethylamino)propyl]amino]butyl] hydrogen phosphate | HCID 439335 | — |
| 반응물 | Dephospho-coa | HCID 444485 | — |
| 반응물 | Riboflavin | HCID 493570 | — |
| 반응물 | glutathionyl-coenzyme A | HCID 86755643 | — |
| 촉매 | Copper | HCID 23978 | — |
| 촉매 | Copper sulfate pentahydrate | HCID 24463 | — |
| 용매 | Tromethamine hydrochloride | HCID 93573 | — |
| 생성물 | Coenzyme A | HCID 87642 | — |
반응 단계
Reduced nicotinamide adenine dinucleotide phosphate (NADPH), riboflavin, flavin adenine mononucleotide (FMN), flavin adenine dinucleotide (FAD), coenzyme A disulfide, glutathionyl-coenzyme A mixed disulfide, 3'-dephospho coenzyme A, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), lysostaphin, bicinchoninic acid solution, 4% copper sulphate pentahydrate solution, 2',5'-adenosine diphosphate (ADP)-Sepharose®; and phenylmethylsulphonyl fluoride (PMSF) were from Sigma (Mississauga, ON). 3'-dephospho CoA was oxidized to the disulfide by incubation at room temperature overnight in Tris-HCl (20 mM), pH 9.0, containing copper (5 μM). 4,4'-phosphopantetheine was formed by incubation of CoA with nucleotide pyrophosphatase. The thiol was oxidized as described above, and the disulfide was purified by high performance liquid chromatography (HPLC). All other chemicals were of reagent grade or better and were used without further purification.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 특허 번호 | US06107068 |
|---|
| 출처 데이터셋 | uspto-grants-2000_08 |
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| ORD ID | ord-20e171cb10bc4349a82014739271f945 |
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| 출처 메모 | uspto-grants-2000_08 |
|---|
