2,5-diketo-D-gluconic acid → 2,5-diketo-D-gluconic acid

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The present inventors have now found the fact that the 2,5-diketo-D-gluconic acid producing strain and the 2-keto-L-gulonic acid producing strain can coexist in the same fermentation broth and that the 2,5-diketo-D-gluconic acid produced from D-glucose by the 2,5-diketo-D-gluconic acid producing strain accumulated in a crude state, can be utilized by the 2-keto-L-gulonic acid producing strain without any disadvantages, and that the aforesaid undesired optical isomer which is by-produced along with the end product by the 2-keto-L-gulonic acid producing strain is, actually, not accumulated in the broth; the by-produced 2-keto-D-gluconic acid is converted again into 2,5-diketo-D-gluconic acid by the 2,5-diketo-D-gluconic acid producing strain for the repeated utilization by the 2-keto-L-gulonic acid producing strain.

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