Sodium Chloride → HCID 178446018, 화합물

HRID 1980277

반응식

HRID 1980277 반응식
C([NH:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:28])[CH3:27])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1)(=O)CNC(C1C=CC=CC=1)=O.[Cl-].[Na+].C[C@H](NC([C@H]1N(C([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N)CC(O)=O)=O)CCCNC(N)=N)=O)C(C)C)=O)CC2C=CC(O)=CC=2)=O)C(C)C)=O)CC2NC=NC=2)=O)CCC1)=O)C(O)=O>O>[NH2:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:27])[CH3:28])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1

참가 물질

5

반응물 3

HCID 5234Sodium ChlorideHCID 178445996HCID 178445996, 화합물HCID 178448346H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

생성물 1

HCID 178446018HCID 178446018, 화합물

시약·촉매·용매 1

HCID 962Water용매

실험 절차

50 μl of a solution containing 0.01 mole/liter of hippuryl-histidyl-leucine (substrate) and 0-100 μl of a test compound solution were added to 300 μl of a 0.2M tris-hydrochloric acid buffer solution containing 0.2 mole/liter of sodium chloride. The total volume of said mixture was adjusted to 450 μl with water. Then, 50 μl of angiotensin-converting enzyme (ACE) isolated from pig's renal cortex were added to the mixture, and the mixture was allowed to stand at 37° C. for 20 minutes. The amount of histidyl-leucine produced from the substrate by the action of ACE was assayed microbiologically by the use of Leuconostoc mesenteroides P-60, and the ACE-inhibitory activity of the test compound was estimated therefrom. The results are shown in the following Table 1.

후처리

  1. customisolated from pig's renal cortex
  2. additionwere added to the mixture

출처 및 근거

ORD 기록
ord-e82be9ce65c8433cb41cf69a0fcbdf35
출처 데이터셋
uspto-grants-1985_04
특허 번호
US04508727

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