1,2-Dichloroethane → N-Boc-Tyr(Bn)-Asn-Trp-NHPh

HRID 2074354노트

반응식

HRID 2074354 반응식
[NH2:1][C@H:2]([C:13]([NH:15][C:16]1[CH:21]=[CH:20][CH:19]=[CH:18][CH:17]=1)=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1.[NH:22]([C:50]([O:52][C:53]([CH3:56])([CH3:55])[CH3:54])=[O:51])[C@H:23]([C:39]([NH:41][C@H:42]([C:47](O)=[O:48])[CH2:43][C:44](=[O:46])[NH2:45])=[O:40])[CH2:24][C:25]1[CH:30]=[CH:29][C:28]([O:31][CH2:32][C:33]2[CH:38]=[CH:37][CH:36]=[CH:35][CH:34]=2)=[CH:27][CH:26]=1.C(Cl)CCl.C1C=CC2N(O)N=NC=2C=1>C(Cl)Cl.CN(C=O)C>[NH:22]([C:50]([O:52][C:53]([CH3:56])([CH3:55])[CH3:54])=[O:51])[C@H:23]([C:39]([NH:41][C@H:42]([C:47]([NH:1][C@H:2]([C:13]([NH:15][C:16]1[CH:21]=[CH:20][CH:19]=[CH:18][CH:17]=1)=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1)=[O:48])[CH2:43][C:44](=[O:46])[NH2:45])=[O:40])[CH2:24][C:25]1[CH:30]=[CH:29][C:28]([O:31][CH2:32][C:33]2[CH:38]=[CH:37][CH:36]=[CH:35][CH:34]=2)=[CH:27][CH:26]=1

참가 물질

6

반응물 4

HCID 111,2-DichloroethaneHCID 757711-HydroxybenzotriazoleHCID 16735217(S)-2-Amino-3-(1H-indol-3-yl)-N-phenylpropanamideHCID 57611773N-Boc-Tyr(Bn)-Asn-OH

생성물 1

HCID 16735715N-Boc-Tyr(Bn)-Asn-Trp-NHPh수율 30.1%

시약·촉매·용매 1

HCID 22350042dichloromethane N,N-dimethylformamide용매

실험 절차

compound SP314C2. Same procedure as above with Trp-NHPh (102.9 mg, 0.368 mmol), N-Boc-Tyr(Bn)-Asn-OH (178.9 mg, 0.368 mmol), EDC (78.2 mg, 0.41 mmol) and HOBt (55 mg, 0.41 mmol) in CH2Cl2/DMF (1.5 mL, 1/1). The crude residue was purified by flash column chromatography on silica gel (0-5% MeOH/CH2Cl2) to give an off-white solid (82.8 mg, 30%). 1H NMR (300 MHz, DMSO-d6) δ 1.28 (s, 9H, (CH3)3), 2.57-3.26 (m, 6H, CH2 Tyr, CH2 Trp, CH2 Asn), 4.11 (m, 1H, CHα), 4.58 (m, 2H, 2 CHα), 5.03 (broad s, 2H, CH2 (Bn)), 6.86-7.65 (m, 22H), 8.2 (m, 2H), 9.82 (s, 1H), 10.78 (s, 1H). 13C NMR (75 MHz, DMSO-d6) δ 27.3 (CH2 Trp), 28.1 ((CH3)3), 36.4, 37 (CH2 Tyr, CH2 Asn), 49.5, 54.5, 55.7 (CHα Tyr, Asn, Trp), 69 (CH2 (Bn)), 78.1 (C(CH3)3), 109.7, 110, 111.2, 114.2, 118.2, 119.6, 120.8, 123.4, 123.6, 127.2, 127.5, 127.7, 128.3, 128.5, 130.1, 136, 137.2, 138.7 (25 aromatic C), 155.2, 156.8 (Car-O, CO carbamate), 170, 171, 171.7, 171.9 (4 CO amide). Anal. Calcd. for C42H46N6O7: C, 67.54; H, 6.21; N, 11.25. Found: C, 67.14; H, 6.27; N, 11.27.

후처리

  1. customThe crude residue was purified by flash column chromatography on silica gel (0-5% MeOH/CH2Cl2)

출처 및 근거

ORD 기록
ord-0f4955c96ad64de48e4ec0aa5bce6d20
출처 데이터셋
uspto-grants-2011_04
특허 번호
US07919468B2

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