N,N,N-trimethylglycinium → Carnitine Chloride

HRID 2081943

반응식

HRID 2081943 반응식
[CH3:1][N+:2]([CH2:5][C:6]([OH:8])=O)([CH3:4])[CH3:3].C[N+](CC[CH2:15][C:16]([O-:18])=[O:17])(C)C.[Cl-:19]>>[CH3:1][N+:2]([CH2:5][CH:6]([OH:8])[CH2:15][C:16]([OH:18])=[O:17])([CH3:4])[CH3:3].[Cl-:19]

참가 물질

3

반응물 2

HCID 248N,N,N-trimethylglyciniumHCID 131717742gamma-Butyrobetaine chloride

생성물 1

HCID 5970Carnitine Chloride수율 99.2%

반응 조건

온도
4 °C

실험 절차

A fermentor equipped for continuous culture which contained 1.5 l of a vitamin containing mineral medium (according to Example 1) with 1.5 percent of betaine and 1.0 percent of γ-butyrobetaine-chloride was inoculated with 150 ml of strain 13 (DSM 2903) preculture of the same medium. After 20 hours of aerobic cultivation at 30° C. and pH 7.0, the culture had grown up and the continuous operation was started at a flow rate of 0.1 l/h. The culture solution flowing out of the fermentor was caught in a vessel cooled at 4° C. the cells were removed by centrifugation. According to enzymatic analysis, the supernatant contained 8.8 g of L-carnitine per 1 of culture. This corresponded to a yield of 99.2 percent (proven analytically), calculated on the concentration of the γ-butyrobetaine-chloride used. The solid L-carnitine chloride was isolated from the solution by means of ion chromatography and water separation.

후처리

  1. customA fermentor equipped for continuous culture which
  2. customwas caught in a vessel
  3. customwere removed by centrifugation

출처 및 근거

ORD 기록
ord-75a443396b264b738133850d994a2aa1
출처 데이터셋
uspto-grants-1993_02
특허 번호
US05187093

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