Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Pyruvate → 9-Azido-9-deoxy-N-acetylneuraminic acid
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Pyruvate | HCID 107735 | — |
| 반응물 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 반응물 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 반응물 | 6-azido-6-deoxy-N-acetylmannosamine | HCID 11708572 | — |
| 용매 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 생성물 | 9-Azido-9-deoxy-N-acetylneuraminic acid | HCID 15479030 | 84% |
반응 단계
In a 10 mL of 0.1M potassium phosphate buffer (pH 7.5) containing 10 mM DTT and 0.5M pyruvate were dissolved 50 mg of 6-azido-6-deoxy-N-acetylmannosamine and 1.5 mg of NeuAc aldolase. The starting material was consumed in 14 hours. The solution was lyophilized and the purification was carried out with Bio Gel P-2 gel filtration (3×90 cm) chromatography with a flow rate of 6 mL/40 minutes, at 4° C. The fractions containing the product were pooled and freezed dry to give Compound 203 in 84 percent yield.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US05593887 |
| 출처 데이터셋 | uspto-grants-1997_01 |
| ORD ID | ord-20091454213a4006a46762a1aa23be0d |
| 출처 메모 | uspto-grants-1997_01 |