참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Acetate | HCID 175 | — |
| 반응물 | Phosphate | HCID 1061 | — |
| 반응물 | Borate | HCID 26574 | — |
| 반응물 | Carbonate bicarbonate | HCID 86586141 | — |
| 반응물 | Glycine sodium hydroxide | HCID 86654294 | — |
| 반응물 | Veronal sodium acetate-HCl | HCID 131719622 | — |
| 생성물 | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
반응 단계
Any buffer providing the desired pH which does not interfere the measurement of enzyme activity is suitable for use in the present invention. However, more specifically, a conventional acetate buffer can be used to provide a pH of 6.0 or less; a conventional phosphate buffer can be used to provide a pH of from 5.5 to 9.5; a carbonate-bicarbonate buffer (e.g., K2CO3 --NaHCO3) buffer can be used to provide a pH of from 8.5 to 11.0; a conventional Britton-Robinson buffer can be used to provide a pH of from 5.0 to 9.0; a conventional Veronal-sodium acetate-HCl buffer can be used to provide a pH of from 3.0 to 9.0; a TRIS-HCl buffer can be used to provide a pH of from 6.8 to 8.5; a conventional glycine-NaOH buffer can be used to provide a pH of from 8.5 to 9.5; a conventional borate buffer can be used to provide a pH of from 8.0 to 11.0; a conventional EDTA buffer can provide a pH of about 8.5; etc.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| 특허 번호 | US05595867 |
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| 출처 데이터셋 | uspto-grants-1997_01 |
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| ORD ID | ord-6b3c35f6f848441c8a2ccfd049611ed2 |
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| 출처 메모 | uspto-grants-1997_01 |
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