Ethylenediamine → 2-(1-methyl-1,4,5,6,7,8-hexahydrocycloheptapyrazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid tert-butylamide

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To a solution of 2-(1-methyl-1,4,5,6,7,8-hexahydrocycloheptapyrazol-3-yl)-5-(2-trimethylsilanyl-ethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid tert-butylamide (40 mg, 0.08 mmol) in CH2Cl2 (2 mL) was added TFA (1.0 mL, 13.0 mmol). The yellow reaction mixture was stirred at room temperature for 3 h then concentrated. The residue was redissolved in CH2Cl2 (2 mL) and ethylenediamine (0.1 mL, 1.48 mmol) was added. The reaction mixture was stirred at room temperature for 0.5 h then concentrated and directly purified by silica gel chromatography with 0% to 3% MeOH/EtOAc to isolate 20 mg (68%) of 2-(1-methyl-1,4,5,6,7,8-hexahydrocycloheptapyrazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid tert-butylamide as an off-white solid. MS: (M+Na)+=389; 1H NMR (DMSO-d6, 300 MHz): δ (ppm) 12.63 (br. s., 1H), 8.80 (s, 1H), 8.27 (s, 1H), 7.83 (s, 1H), 3.81 (s, 3H), 3.02-3.12 (m, 2H), 2.74-2.83 (m, 2H), 1.82 (br. s., 2H), 1.57-1.73 (m, 4H), 1.44 (s, 9H).

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