Trifluoroacetic acid → N-((3R,4R)-3-aminotetrahydro-2H-pyran-4-yl)-2-(5-methoxy-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide

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To a pale yellow solution of tert-butyl (3R,4R)-4-(2-(5-methoxy-1-methyl-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamido)tetrahydro-2H-pyran-3-ylcarbamate (115 mg, 176 μmol) in dichloromethane (3.00 mL) was added TFA (1.48 g, 1.00 mL, 13.0 mmol, Eq: 73.6), the reaction mixture turned dark red and was stirred at 25° C. overnight then concentrated. The residue was re-dissolved in 5 mL of a solution of (dichloromethane/MeOH/ammonium hydroxide; 60:10:1) and stirred at 25° C. for 3 h, then evaporated to a light yellow solid, dissolved in dichloromethane (added few drops MeOH and heating) and purified by chromatography (60 g column, 50 μm from Analogix, 0 to 5% over 15 min (MeOH containing 10% ammonium hydroxide)/dichloromethane. The pure product dissolved in dichloromethane/MeOH and most dichloromethane evaporated for solid formation, solid was separated by decantation and dried under high vacuum to give N-((3R,4R)-3-aminotetrahydro-2H-pyran-4-yl)-2-(5-methoxy-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (48 mg, 114 μmol, 64.6%) as an off-white solid. MS (M+H)+=422 (100% purity); 1H NMR (DMSO-d6) δ: 9.05 (s, 1H), 8.45 (d, J=8.3 Hz, 1H), 8.39 (s, 1H), 8.17 (d, J=2.6 Hz, 1H), 7.70 (d, J=9.1 Hz, 1H), 7.31 (dd, J=9.1, 2.3 Hz, 1H), 4.23 (td, J=7.6, 4.0 Hz, 1H), 4.14 (s, 3H), 4.08 (q, J=5.3 Hz, 1H), 3.87 (s, 3H), 3.84 (br. s., 1H), 3.52-3.72 (m, 2H), 3.38-3.51 (m, 1H), 3.15 (d, J=4.9 Hz, 2H), 2.88 (br. s., 1H), 1.85-2.07 (m, 1H), 1.70 (d, J=11.7 Hz, 1H).

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