반응식
[OH:1][C:2]1[CH:3]=[C:4]([CH:11]=[CH:12][C:13]=1[OH:14])[CH2:5][C@@H:6]([C:8]([OH:10])=[O:9])[NH2:7]>P([O-])([O-])([O-])=O>[CH2:5]1[C:4]2[C:11](=[CH:12][C:13]([C:2]([CH:3]=2)=[O:1])=[O:14])[NH:7][CH:6]1[C:8]([OH:10])=[O:9]참가 물질
3
반응물 1
생성물 1
시약·촉매·용매 1
실험 절차
To a 96-well plate, 40 μl of mushroom-derived tyrosinase (125 unit/ml; dissolved in a 67 mmol/l phosphate buffer solution; Sigma), 120 μl of 3,4-dihydroxyphenylalanine that serves as a substrate (L-dopa; 5 mmol/l; dissolved in a 67 mmol/l phosphate buffer solution; Sigma), and 40 μl of an inhibitor solution were added. The plate was allowed to stand for 37° C. for 30 minutes. Then, the amount of dopachrome produced was determined by absorbance at 490 nm. Tyrosinase inhibitory activity was expressed as an inhibitory rate obtained by the following equation: Inhibitory Rate (%)=[(A−B)−(C−D)]/(A−B)×100
출처 및 근거
- ORD 기록
- ord-44e11de5cdfd4e08be52342d10bd0da0
- 출처 데이터셋
- uspto-grants-2011_01
- 특허 번호
- US07863248B2
연결된 노트
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