Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Hydrochloric Acid → Phthalaldehyde
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Hydrochloric Acid | HCID 313 | — |
| 반응물 | L-Phenylalanine | HCID 6140 | — |
| 반응물 | tert-Butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanyl-OH | HCID 132822 | — |
| 반응물 | o-Phthalaldehyde 2-mercaptoethanol | HCID 88526415 | — |
| 용매 | Methanol | HCID 887 | — |
| 생성물 | Phthalaldehyde | HCID 4807 | — |
반응 단계
Four aliquots of the conjugate were hydrolyzed with concentrated HCl at 115° C. for 20 hours and analyzed for phenylalanine. The phenylalanine was quantified as the o-phthalaldehyde/2-mercaptoethanol derivative using reverse phase HPLC with a fluorescence detector (HPLC conditions as Example 24 except the eluent gradient was 12% to 80% methanol over 20 min. and then held at 80% for 5 min.). The o-phthalaldehyde derivative was prepared as given in Example 24. For a 25 μL aliquot of the conjugate, 26 nmoles phenylalanine were found. This corresponds to 0.26 μmoles BOCPheLeuPheLeuPhe on the conjugate.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US05527524 |
| 출처 데이터셋 | uspto-grants-1996_06 |
| ORD ID | ord-db97cb5f6c2b40ada0f48fcec6dc3a50 |
| 출처 메모 | uspto-grants-1996_06 |