4-Nitrophenol → Barbituric Acid

HRID 26690

반응식

HRID 26690 반응식
C([C:4]1(C(C)CC(O)=O)[C:9](=[O:10])[NH:8][C:7](=[O:11])[NH:6][C:5]1=[O:12])C=C.C1(N=C=NC2CCCCC2)CCCCC1.C1C([N+]([O-])=O)=CC=C(O)C=1>CN(C)C=O>[NH:6]1[C:5](=[O:12])[CH2:4][C:9](=[O:10])[NH:8][C:7]1=[O:11]

참가 물질

5

반응물 3

HCID 9804-NitrophenolHCID 10868DicyclohexylcarbodiimideHCID 13023202HCID 13023202, 화합물

생성물 1

HCID 6211Barbituric Acid

시약·촉매·용매 1

HCID 6228Dimethylformamide용매3회

실험 절차

5-Allyl-5-(β-carboxy-α-methyl-ethyl)-barbituric acid (10 mg.) was dissolved in 0.5 ml. dimethyl formamide (DMF) and was treated first with a solution of 5 mg. dicyclohexyl carbodiimide (DCC) in 0.5 ml. DMF and then with a solution of 12 mg. p-nitrophenol in 0.5 ml. DMF at 4° C. After standing overnight at this temperature, the mixture was evaporated to dryness and then dissolved in 1.5 ml. of a 1:1 mixture of glycerine-water. Bovine serum albumin (20 mg.) was added and the mixture was allowed to react for eight hours at room temperature and then overnight at 4° C. The product was then dialyzed against distilled water for two days to remove unreacted barbituric acid derivative and the p-nitrophenol which was displaced from the barbituric acid by the protein, to afford the bovine serum albumin-barbituric acid conjugate. The degree of substitution was estimated to be 2-3 moles of barbiturates per mole of protein, calculated from the extinction coefficient of the absorbtion at 202 mμ.

후처리

  1. customat 4° C
  2. customthe mixture was evaporated to dryness
  3. dissolutiondissolved in 1.5 ml
  4. additionof a 1:1 mixture of glycerine-water
  5. additionBovine serum albumin (20 mg.) was added
  6. customto react for eight hours at room temperature
  7. customovernight
  8. customat 4° C
  9. customThe product was then dialyzed
  10. distillationagainst distilled water for two days
  11. customto remove unreacted barbituric acid derivative

출처 및 근거

ORD 기록
ord-74481810179c4b9dbbb82e033283bb5b
출처 데이터셋
uspto-grants-1979_04
특허 번호
US04151268

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