Pyrophosphoric acid → Guanosine Monophosphate

HRID 276749

반응식

HRID 276749 반응식
[P:1]([O:5]P(O)(O)=O)([OH:4])([OH:3])=[O:2].[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:24])[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16]O)[C@@H:13]([OH:14])[C@H:11]1[OH:12].[C@@H:29]1([N:38]2[C:48]3[N:47]=[C:45]([NH2:46])[NH:44][C:42](=[O:43])[C:41]=3[N:40]=[CH:39]2)[O:37][C@H:34]([CH2:35][OH:36])[C@@H:32]([OH:33])[C@H:30]1[OH:31]>C([O-])(=O)C>[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:24])[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16][O:5][P:1]([OH:3])([OH:4])=[O:2])[C@@H:13]([OH:14])[C@H:11]1[OH:12].[C@@H:29]1([N:38]2[C:48]3[N:47]=[C:45]([NH2:46])[NH:44][C:42](=[O:43])[C:41]=3[N:40]=[CH:39]2)[O:37][C@H:34]([CH2:35][O:36][P:1]([OH:4])([OH:3])=[O:2])[C@@H:32]([OH:33])[C@H:30]1[OH:31]

참가 물질

6

반응물 3

HCID 1023Pyrophosphoric acidHCID 135398635GuanosineHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

생성물 2

HCID 135398631Guanosine MonophosphateHCID 178527090HCID 178527090, 화합물

시약·촉매·용매 1

HCID 175Acetate용매

실험 절차

Pyrophosphoric acid (15 g/dl) and 8 g/dl of inosine or guanosine were dissolved in an acetate buffer (pH 4.0). To this was added E. coli JM109 strain having introduced therein each mutant acid phosphatase gene such that the concentration reached 100 mg/dl in terms of the dry cell weight. The reaction was conducted at 50° C. for 9 hours while maintaining the pH at 4.0, and the amount of 5′-inosinic acid or 5′-guanylic acid formed was measured. The results are shown in Table 17. The nucleoside phosphate ester formed was only a nucleoside-5′-phosphate ester, and the production of a nucleoside-2′-phosphate ester and a nucleoside-3′-phosphate ester as by-products was not observed at all. The reaction was also conducted at 30° C. for 12 hours using E. coli JM109/pEPI380 strain as a control. The results are also shown in Table 17.

후처리

  1. additionTo this was added E
  2. additionstrain having introduced
  3. concentrationeach mutant acid phosphatase gene such that the concentration
  4. temperaturewhile maintaining the pH at 4.0

출처 및 근거

ORD 기록
ord-21e6e5ab5fcc4010bb668be326a5c704
출처 데이터셋
uspto-grants-2002_03
특허 번호
US06355472B2

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