Sodium Bicarbonate → 5-(2-(4-Hydroxyphenyl)Ethenyl)Benzene-1,3-Diol

HRID 277304

반응식

HRID 277304 반응식
C([O-])(O)=O.[Na+].C[C@@H](N)[C@H]1O[C@H]([O:14][C@H:15]2[C@H:20](O)[C@@H:19]([O:22][C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H:18](N)[CH2:17][C@@H:16]2N)[C@H](N)CC1.C[C@@H](NC)[C@H]1O[C@H](O[C@H:47]2[C@H:52](O)[C@@H:51]([O:54][C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H:50](N)[CH2:49][C@@H:48]2N)[C@H](N)CC1.[CH3:71][C@@:72]1(O)[C@H](NC)[C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@H](CN)CC[C@H]3N)[C@@H](N)C[C@H]2N)OC1.OS(O)(=O)=O>>[C:17]1([CH:71]=[CH:72][C:48]2[CH:47]=[CH:52][C:51]([OH:54])=[CH:50][CH:49]=2)[CH:16]=[C:15]([OH:14])[CH:20]=[C:19]([OH:22])[CH:18]=1

참가 물질

3

반응물 2

HCID 516892Sodium BicarbonateHCID 6419933Gentamicine sulfate

생성물 1

HCID 50565-(2-(4-Hydroxyphenyl)Ethenyl)Benzene-1,3-Diol

실험 절차

Cultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md., were grown to confluence in Medium 199 supplemented with 5% fetal bovine serum, 0.075% NaHCO3, and 50 μg/ml gentamycin sulfate in 25 cm2 tissue culture flasks. Cells were infected with pseudorabies virus at a multiplicity of infection (moi) of one and incubated at room temperature for one hour to allow for virus attachment to and penetration of the cell. Under these conditions, approximately half of the cells are infected with virus. Thereafter, the cultures were rinsed three time with media and incubated in medium containing resveratrol at a final concentration of 50 μg/ml. Stock solutions of the resveratrol, obtained from Sigma Chemical Co, St. Louis, Mo. were prepared in 100% ethanol and diluted to the final concentration in tissue culture media. The maximum concentration of alcohol in the medium was 0.5%. Controls were treated identically, but were incubated without resveratrol.

후처리

  1. customCultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md
  2. washThereafter, the cultures were rinsed three time with media
  3. additioncontaining resveratrol at a final concentration of 50 μg/ml
  4. customwere prepared in 100% ethanol
  5. additiondiluted to the final
  6. concentrationconcentration in tissue culture media
  7. concentrationThe maximum concentration of alcohol in the medium
  8. additionControls were treated identically

출처 및 근거

ORD 기록
ord-7433d0a52533498fa8c30a4adea1659d
출처 데이터셋
uspto-grants-2002_03
특허 번호
US06355692B2

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