Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Potassium Chloride → 2,5-Dihydrofuran-3,4-dicarboxylic acid
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Potassium Chloride | HCID 4873 | — |
| 반응물 | Dichlorophenol Indophenol | HCID 13726 | — |
| 반응물 | X 100 | HCID 19153 | — |
| 반응물 | Dihydroorotate | HCID 5461056 | — |
| 용매 | Tromethamine hydrochloride | HCID 93573 | — |
| 생성물 | 2,5-Dihydrofuran-3,4-dicarboxylic acid | HCID 6420982 | — |
반응 단계
The standard assay mixture contained 50 μM decyclo ubichinone, 100 μM dihydroorotate, 60 μM 2,6-dichloroindophenol, as well as 20 mU DHODH. The volume activity of the recombinant enzyme used was 30 U/ml. Measurements were conducted in 50 mM TrisHCl (150 mM KCl, 0,1% Triton X-100, pH. 8,0) at 30° C. in a final volume of 1 ml. The components were mixed, and the reaction was started by adding dihydroorotate. The course of reaction was followed by spectrophotometrically measuring the decrease in absorption at 600 nm for 2 min.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07423057B2 |
| 출처 데이터셋 | uspto-grants-2008_09 |
| ORD ID | ord-8c87408e6e1e4db5a86984764b0046b0 |
| 출처 메모 | uspto-grants-2008_09 |