Water → N,N'-Dicyclohexylurea

HRID 32339

반응식

HRID 32339 반응식
[CH:1]1([N:7]=[C:8]=[N:9][CH:10]2[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]2)[CH2:6][CH2:5][CH2:4][CH2:3][CH2:2]1.[OH2:16]>CN(C=O)C>[C:8]([NH:7][CH:1]1[CH2:2][CH2:3][CH2:4][CH2:5][CH2:6]1)([NH:9][CH:10]1[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]1)=[O:16]

참가 물질

4

반응물 2

HCID 962WaterHCID 10868Dicyclohexylcarbodiimide

생성물 1

HCID 4277N,N'-Dicyclohexylurea

시약·촉매·용매 1

HCID 6228Dimethylformamide용매

반응 조건

온도
4 °C

실험 절차

To N-TeocT4 (0.017 g, 17 μmol) in 1 mL dry DMF was added dicyclohexylcarbodiimide (0.004 g, 18 μmol). After stirring for 30 minutes N-dimethyl-4-aminopyridine (0.004 g, 36 μmol) and Gly18 (0.017 g, 17 μmol) were added and the reaction stirred overnight. The cloudy solution was poured into 20 mL H2O and extracted twice with 10 mL CH2Cl2. The aqueous component was acidified to pH 3 with 1 N HCl and chilled to 4° C. The material was isolated by centrifugation and the pellet washed 3 times with 8 mL H2O. The pellet was dried in vacuum to yield dicyclohexylurea and N-TeocT4-Gly18. (SEQ ID NO: 6)

후처리

  1. additionGly18 (0.017 g, 17 μmol) were added
  2. extractionextracted twice with 10 mL CH2Cl2
  3. customThe material was isolated by centrifugation
  4. washthe pellet washed 3 times with 8 mL H2O
  5. customThe pellet was dried in vacuum

출처 및 근거

ORD 기록
ord-d889715e4eb84c7d91566f2edec9a1ef
출처 데이터셋
uspto-grants-2008_09
특허 번호
US07427600B2

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