Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Pyridoxal 5'-phosphate → Indole
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Pyridoxal 5'-phosphate | HCID 1051 | — |
| 반응물 | L-Tryptophan | HCID 6305 | — |
| 반응물 | Trichloroacetic Acid | HCID 6421 | — |
| 반응물 | Monopotassium phosphate dipotassium phosphate | HCID 21889592 | — |
| 생성물 | Indole | HCID 798 | — |
반응 단계
Measured in accordance with the method of Yamada et al. as follows. In 4.0ml of a reaction mixture containing 10 micromoles of L-tryptophan, 0.4 micromole of pyridoxalphosphate (PLP)), 200 micromoles of K2HPO4 -KH2PO4 (pH 8.0), the enzyme is incubated at 65° C. for 10 minutes. Then, 1.0 ml of a 30% aqueous solution of trichloroacetic acid (TCA) is added to stop the reaction. Indole formed in the reaction system is quantitatively determined by the method of E. McEvoy-Bowe [The ANALYST, vol. 88, pages 893-894 (1963)]. The activity is expressed in units in which 1 U is the amount in micromoles of indole formed during 1 minute.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US04761374 |
| 출처 데이터셋 | uspto-grants-1988_08 |
| ORD ID | ord-206ec9e44fcb496288f80b4c23c1714c |
| 출처 메모 | uspto-grants-1988_08 |