Triethylamine → L-tyrosyl-D-alanyl-glycyl-L-phenylalanyl-L-proline ethylamide

HRID 358316노트

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HRID 358316 반응식
[NH:1](C(OCC1C=CC=CC=1)=O)[C@H:2]([C:11]([NH:13][C@@H:14]([C:16]([NH:18][CH2:19][C:20]([NH:22][C@H:23]([C:31]([N:33]1[CH2:40][CH2:39][CH2:38][C@H:34]1[C:35](O)=[O:36])=[O:32])[CH2:24][C:25]1[CH:30]=[CH:29][CH:28]=[CH:27][CH:26]=1)=[O:21])=[O:17])[CH3:15])=[O:12])[CH2:3][C:4]1[CH:9]=[CH:8][C:7]([OH:10])=[CH:6][CH:5]=1.Cl.[CH2:52]([NH2:54])[CH3:53].C(N(CC)CC)C.C1(N=C=NC2CCCCC2)CCCCC1>CN(C)C=O>[CH2:52]([NH:54][C:35](=[O:36])[C@@H:34]1[CH2:38][CH2:39][CH2:40][N:33]1[C:31](=[O:32])[C@H:23]([CH2:24][C:25]1[CH:26]=[CH:27][CH:28]=[CH:29][CH:30]=1)[NH:22][C:20](=[O:21])[CH2:19][NH:18][C:16](=[O:17])[C@@H:14]([CH3:15])[NH:13][C:11](=[O:12])[C@H:2]([CH2:3][C:4]1[CH:5]=[CH:6][C:7]([OH:10])=[CH:8][CH:9]=1)[NH2:1])[CH3:53]

참가 물질

6

반응물 4

HCID 8471TriethylamineHCID 10868DicyclohexylcarbodiimideHCID 11198Ethylamine hydrochlorideHCID 131713663HCID 131713663, 화합물

생성물 1

HCID 10371011L-tyrosyl-D-alanyl-glycyl-L-phenylalanyl-L-proline ethylamide

시약·촉매·용매 1

HCID 6228Dimethylformamide용매

실험 절차

0.7 g. (1 mmoles) of Z-Tyr-D-Ala-Gly-Phe-Pro-OH (3rd step of Example 5) are dissolved in 2 ml. of dimethyl formamide, then 0.1 g. (1.24 mmoles) of ethylamine hydrochloride, 0.17 ml. (1.24 mmoles) of triethylamine and 0.21 g. (1 mmole) of dicyclohexyl-carbodiimide are added. The reaction mixture is allowed to stand for one day, filtered and diluted with 30 ml. of ethyl acetate. The solution is washed with 0.5N sulphuric acid, water, 5% solution of sodium hydrogen carbonate and water, then dried and evaporated. The residue on evaporation (Rf2 0.80-0.85) is dissolved in 30 ml. of methanol and hydrogenated in the presence of palladium as catalyst. At the end of the reaction the catalyst is filtered off, the solution evaporated, rubbed with ether, filtered, washed with ether and dried. Yield: 0.43 g. (75%) of the named pentapeptide; Rf4 0.34-0.44.

후처리

  1. filtrationfiltered
  2. additiondiluted with 30 ml
  3. washThe solution is washed with 0.5N sulphuric acid, water, 5% solution of sodium hydrogen carbonate and water
  4. customdried
  5. customevaporated
  6. customThe residue on evaporation (Rf2 0.80-0.85)
  7. dissolutionis dissolved in 30 ml
  8. customAt the end of the reaction the catalyst
  9. filtrationis filtered off
  10. customthe solution evaporated
  11. filtrationfiltered
  12. washwashed with ether
  13. customdried

출처 및 근거

ORD 기록
ord-fe3518b652c9494288ad6d4e01910fac
출처 데이터셋
uspto-grants-1984_08
특허 번호
US04465625

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