Phosphate → HCID 178508926, 화합물

HRID 373662

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HRID 373662 반응식
[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[N:14]=[C:15]([NH2:19])[N:16]=[C:17](N)[C:3]=2[N:2]=1.P([O-])([O-])([O-])=[O:21]>[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O>[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[NH:14][C:15]([NH2:19])=[N:16][C:17](=[O:21])[C:3]=2[N:2]=1

참가 물질

4

반응물 2

HCID 1061PhosphateHCID 4515592-Aminooxetanocin A

생성물 1

HCID 178508926HCID 178508926, 화합물

시약·촉매·용매 1

HCID 6083Adenosine Monophosphate용매

반응 조건

온도
37 °C

실험 절차

After 2.0 g of 2-amino-OXT-A was dissolved in 300 ml of 1/10M phosphate buffer (pH 6.5), 200 units of 5'-adenylic acid deaminase (manufactured by Sigma Co., A-1907) were added to the solution followed by stirring at 37° C. for 50 hours. After the reaction solution was passed through a column packed with MCI® GEL CHP 20 (300 ml) and the reaction products were adsorbed onto it, the products were eluted by water. Fractions showing an Rf value of about 0.42 in silica gel TLC [developing solvent: n-butanol-acetic acid-water (4:1:2)] were collected and concentrated to dryness under reduced pressure to give 1.8 g of OXT-G as colorless crystals (yield, 90%).

후처리

  1. addition, A-1907) were added to the solution
  2. washthe products were eluted by water
  3. customwere collected
  4. concentrationconcentrated to dryness under reduced pressure
  5. customto give

출처 및 근거

ORD 기록
ord-b18c4afd2c6c4adb8bfac8575dfa5d7b
출처 데이터셋
uspto-grants-1992_11
특허 번호
US05164500

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