Adenosine Monophosphate → HCID 178508926, 화합물

HRID 373663

반응식

HRID 373663 반응식
[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[N:14]=[C:15]([NH2:19])[N:16]=[C:17](N)[C:3]=2[N:2]=1.[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1[OH:22]>P([O-])([O-])([O-])=O>[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[NH:14][C:15]([NH2:19])=[N:16][C:17](=[O:22])[C:3]=2[N:2]=1

참가 물질

4

반응물 2

HCID 6083Adenosine MonophosphateHCID 4515592-Aminooxetanocin A

생성물 1

HCID 178508926HCID 178508926, 화합물

시약·촉매·용매 1

HCID 1061Phosphate용매

반응 조건

온도
37 °C

실험 절차

After 2.0 g of 2-amino-OXT-A was dissolved in 300 ml of 1/10M phosphate buffer (pH 6.5), 8.0 g of 5'-adenylic acid deaminase (manufactured by Amano Pharmaceutical Co., Ltd., DEAMIZYME®) was added to the solution followed by stirring at 37° C. for 70 hours. After insoluble matters of the reaction solution were removed by filtration, the filtrate was passed through a column packed with 50 ml of activated charcoal powders (manufactured by Wako Pure Chemical Industry K.K., for chromatography). After washing with water, elution was performed with 50% aqueous methanol. By concentrating the desired fraction to dryness, crude OXT-G was obtained. The crude powders were dissolved in 50 ml of water and the OXT-G in the solution was adsorbed onto MCI® GEL CHP 20 (300 ml) followed by elution with water. The desired fraction was concentrated and evaporated to dryness under reduced pressure to give 1.5 g of OXT-G as colorless crystals (yield, 75%).

후처리

  1. additionwas added to the solution
  2. customAfter insoluble matters of the reaction solution were removed by filtration
  3. washAfter washing with water, elution
  4. concentrationBy concentrating the desired fraction to dryness

출처 및 근거

ORD 기록
ord-152f04f6ff8c402b83461295500c4937
출처 데이터셋
uspto-grants-1992_11
특허 번호
US05164500

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