Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → Ribosylthymidine diphosphate
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | 5-Methyluridine 5'-triphosphate | HCID 451388 | — |
| 반응물 | Pyrophosphate | HCID 644102 | — |
| 반응물 | Triphosphate | HCID 3440921 | — |
| 용매 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 용매 | Ammonium Sulfate | HCID 6097028 | — |
| 생성물 | Ribosylthymidine diphosphate | HCID 23379797 | — |
반응 단계
5-Methyluridine 5'-triphosphate (0.0047 g, 0.009 mMol) was dissolved in 0.7 mL 1M Tris buffer (pH 8.5 containing 1M MgCl2) then 0.3 mL of a 0.25M glucose solution in 1M Tris buffer (pH 8.5 containing 1M MgCl2) added. A T0 timepoint was taken by reverse phase anion exchange HPLC. Hexokinase (EC 2.7.1.1, Boehringer Mannheim, from yeast overproducer), 75 units in 3.2M ammonium sulfate suspension, was added to the reaction which was incubated at 25° C. An HPLC was taken at 66 hours indicating complete conversion to diphosphate with no triphosphate remaining. Triphosphate retention: 17.5 minutes; diphosphate retention: 10.3 minutes.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US06022527 |
| 출처 데이터셋 | uspto-grants-2000_02 |
| ORD ID | ord-68d8982572b845319114b9e406e5f401 |
| 출처 메모 | uspto-grants-2000_02 |