반응식
[OH:1][CH2:2][CH2:3][CH2:4][C:5]([O-:7])=[O:6].[OH:8][CH2:9][CH2:10][C:11]([O-:13])=[O:12].[CH3:14][C:15]1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)[C:21](=[O:22])N2[C@H:16]1[C:34]([OH:36])=[O:35].C1C([C@@H:44]([OH:54])[C@H:45](NC(C(Cl)Cl)=O)[CH2:46][OH:47])=CC=C([N+]([O-])=O)C=1>>[OH:1][CH2:2][CH2:3][CH2:4][C:5]([O-:7])=[O:6].[OH:8][CH2:9][CH2:10][C:11]([O-:13])=[O:12].[C:34]([O-:36])(=[O:35])[CH:16]([CH3:15])[OH:47].[CH3:14][O:6][C:5](=[O:7])[CH2:4][CH2:3][CH2:2][OH:1].[CH3:21][O:22][C:44](=[O:54])[CH2:45][CH2:46][OH:47].[CH3:2][O:1][C:46](=[O:47])[CH:45]([CH3:44])[OH:8]참가 물질
반응물 4
생성물 4
실험 절차
4-Hydroxybutyrate-3-hydroxypropionate-lactate terpolymer was prepared according to the method of example 2 except that the collected cells was anaerobically cultured for 3 days in MR medium further containing 2 g/L of 4-hydroxybutyrate (4-HB), 2 g/L of 3-hydroxypropionate (3-HP), 100 mg/L of ampicillin and 30 mg/L of chloramphenicol instead of MR medium further containing g/L of 4-HB, 100 mg/L of ampicillin and 30 mg/L of chloramphenicol. —As a result of the analysis, methyl-4-hydroxybutyrate, methyl-3-hydroxypropionate and methyl-lactate were detected in E. coli Top10/pPs619C1300-CPPCT/pMCSPtbBuk transformant, which meant that new 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer [poly(4-hydroxybutyrate-co-3-hydroxypropionate-co-lactate)] was prepared by the recombinant E. Coli. 1H-NMR and GC-MSD results of the obtained 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer were shown in FIGS. 6 and 7, respectively.
출처 및 근거
- ORD 기록
- ord-2c4161ef867a4cf4a9cfaee7a1d65950
- 출처 데이터셋
- uspto-grants-2013_02
- 특허 번호
- US08383379B2
연결된 노트
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