참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Thiamine | HCID 1130 | — |
| 반응물 | L-Isoleucine | HCID 6306 | — |
| 반응물 | Calcium Carbonate | HCID 10112 | — |
| 반응물 | Sulfuric acid magnesium salt (1:1) | HCID 24083 | — |
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | Monobasic potassium phosphate | HCID 516951 | — |
| 반응물 | Ammonium Sulfate | HCID 6097028 | — |
| 생성물 | L-Glutamic Acid | HCID 33032 | — |
반응 단계
Both strains, VL334thrC+ and VL334thrC-Δtarget gene, can be grown for 18-24 hours at 37° C. on L-agar plates. Then, one loop of the cells can be transferred into test tubes containing 2 ml of fermentation medium. The fermentation medium contains glucose (60 g/l), ammonium sulfate (25 g/l), KH2PO4 (2 g/l), MgSO4 (1 g/l), thiamine (0.1 mg/ml), L-isoleucine (70 μg/ml), and CaCO3 (25 g/l). The pH is adjusted to 7.2. Glucose and CaCO3 are sterilized separately. Cultivation can be carried out at 30° C. for 3 days with shaking. After the cultivation, the amount of L-glutamic acid which is produced can be determined by paper chromatography (liquid phase composition of butanol-acetic acid-water=4:1:1) with subsequent staining by ninhydrin (1% solution in acetone) and further elution of the compounds in 50% ethanol with 0.5% CdCl2.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 특허 번호 | US08088606B2 |
|---|
| 출처 데이터셋 | uspto-grants-2012_01 |
|---|
| ORD ID | ord-504a36b2384746ff84d13f0eadfc2633 |
|---|
| 출처 메모 | uspto-grants-2012_01 |
|---|
