Acefylline → 2-(1,3-Dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-purin-7-yl)-n-[4-(1-hydroxy-1-methyl-ethyl)-phenyl]-acetamide

To a 0° C. solution of acid 101 (0.32 g, 1.34 mmol) in DMF/DMSO (1:1, 20 mL) was added HATU (0.0.76 g, 2.01 mmol). The reaction was then stirred an additional 15 min at room temperature. The solution was cooled to 0° C. and amine 135 (0.30 g, 2.01 mmol) was added. The reaction was then stirred at rt overnight. The DMF was concentrated in vacuo and water was added to the reaction. The aqueous layer was extracted with EtOAc The ethyl acetate layer was then washed again with water and the organic layer was concentrated in vacuo. The product was purified by flash chromatography on silica gel eluting with EtOAc/hexanes and EtOAc/MeOH to yield 402 as a pure light brown semi solid (0.086 g, 17.5%). MS (APCI): m/z 372.2 [M−H]+ Anal. Calcd. For C18H21N5O4. 0.35 EtOAc: C, 57.93; H, 5.96; N, 17.41. Found C, 58.36; H, 6.14; N, 16.96.

1. temperatureThe solution was cooled to 0° C.
2. stirringThe reaction was then stirred at rt overnight
3. concentrationThe DMF was concentrated in vacuo and water
4. additionwas added to the reaction
5. extractionThe aqueous layer was extracted with EtOAc The ethyl acetate layer
6. washwas then washed again with water
7. concentrationthe organic layer was concentrated in vacuo
8. customThe product was purified by flash chromatography on silica gel eluting with EtOAc/hexanes and EtOAc/MeOH

ORD 기록

ord-baba062cb47f4c34aaf02c30011c1cd1

출처 데이터셋

uspto-grants-2013_09

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US08541423B2