4-Methylumbelliferyl beta-D-glucuronide → 4-Methylumbelliferone

HRID 458504

반응식

HRID 458504 반응식
[CH3:1][C:2]1[C:8]2[CH:9]=[CH:10][C:11]([O:13][C@@H]3O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]3O)=[CH:12][C:7]=2[O:6][C:4](=[O:5])[CH:3]=1>C([O-])([O-])=O.[Na+].[Na+]>[CH3:1][C:2]1[C:8]2[CH:9]=[CH:10][C:11]([OH:13])=[CH:12][C:7]=2[O:6][C:4](=[O:5])[CH:3]=1

참가 물질

3

반응물 1

HCID 915534-Methylumbelliferyl beta-D-glucuronide

생성물 1

HCID 52805674-Methylumbelliferone

시약·촉매·용매 1

HCID 10340Sodium Carbonate용매

실험 절차

To evaluate the activity of various promoters, GUS fluorescence was assayed by the method of Jefferson (1987). Maturing seeds at 17 DAF were homogenized in GUS extraction buffer (50 mM NaPO4 (pH 7.01, 10 mM 2-mercaptoethanol, 10 mM Na2-EDTA, 0.1% SDS, 0.1% Triton X-100). After centrifugation, 10 μl of the supernatant was mixed with 90 μl assay buffer containing 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG). After incubating for one hour at 37° C., 900 μl of 0.2 M Na2CO3 was added to the mixture to terminate the reaction. Values obtained using a fluorometer were compared with those obtained from serial dilutions of 4-methylumbelliferone (4MU). The protein amount was determined using a BIORAD Protein Assay kit, with serum albumin as the standard. Three seeds were assayed for each transgenic plant.

후처리

  1. additionAfter centrifugation, 10 μl of the supernatant was mixed with 90 μl assay
  2. customto terminate
  3. customthe reaction
  4. customValues obtained

출처 및 근거

ORD 기록
ord-2531acde295a4a6a89b31f4c70c2fcb2
출처 데이터셋
uspto-grants-2007_03
특허 번호
US07192774B2

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