Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Udp-Glucose → 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Udp-Glucose | HCID 8629 | — |
| 반응물 | Perchloric Acid | HCID 24247 | — |
| 반응물 | alpha-D-glucose 1-phosphate | HCID 65533 | — |
| 반응물 | Tromethamine hydrochloride | HCID 93573 | — |
| 반응물 | Magnesium Chloride | HCID 5360315 | — |
| 반응물 | AzddU triphosphate | HCID 131717599 | — |
| 생성물 | 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose | HCID 131717598 | — |
반응 단계
To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US05159067 |
| 출처 데이터셋 | uspto-grants-1992_10 |
| ORD ID | ord-3cfdeae288a2409fb130096d830f42b3 |
| 출처 메모 | uspto-grants-1992_10 |