Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Urea → L-Tryptophan
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Urea | HCID 1176 | — |
| 반응물 | Chloramphenicol | HCID 5959 | — |
| 반응물 | Thiamine Hydrochloride | HCID 6202 | — |
| 반응물 | Magnesium Sulfate Heptahydrate | HCID 24843 | — |
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | Biotin | HCID 171548 | — |
| 반응물 | Ammonium Sulfate | HCID 6097028 | — |
| 용매 | L-Threonine | HCID 6288 | — |
| 생성물 | L-Tryptophan | HCID 6305 | — |
반응 단계
After culturing at 30° C. for 2 weeks, about 10,000 colonies resistant to chloramphenicol appeared, which were replicated in threonine-free medium (Thr deficient medium) which contained 2% of glucose, 1% of ammonium sulfate, 0.25% of urea, 0.1% of dihydrogen potassium phosphate, 0.04% of magnesium sulfate heptahydrate, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 200 μg/dl of thiamine hydrochloride and 50 μg/dl of biotin, pH 7.0, 1.8% of agar, and 3 strains which were resistant to chloramphenicol and tryptophan independent were obtained.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US04885245 |
| 출처 데이터셋 | uspto-grants-1989_12 |
| ORD ID | ord-ec4b76b4beb443f0b3ee53df3e72b8aa |
| 출처 메모 | uspto-grants-1989_12 |