Isatoic Anhydride → Anthranilate dATP
반응식
[CH:1]1[N:6]=[C:5]2[N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][P:25]([OH:28])([OH:27])=[O:26])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:29])[CH2:11]3)[CH:8]=[N:9][C:4]2=[C:3]([NH2:30])[N:2]=1.[C:31]12[C:37](=[CH:38][CH:39]=[CH:40][CH:41]=1)[NH:36]C(=O)[O:34][C:32]2=[O:33].C([O-])(=O)C1C(=CC=CC=1)N.P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>>[C:32]([O-:34])(=[O:33])[C:31]1[C:37](=[CH:38][CH:39]=[CH:40][CH:41]=1)[NH2:36].[CH:1]1[N:6]=[C:5]2[N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][P:25]([OH:28])([OH:27])=[O:26])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:29])[CH2:11]3)[CH:8]=[N:9][C:4]2=[C:3]([NH2:30])[N:2]=1참가 물질
4
실험 절차
Anthranilate dATP was prepared from dATP and isatoic anhydride by a procedure similar to that described by Hiratsuka {T. Hiratsuka (1982) J. Biol. Chem 257, pp. 13354-13358} for the synthesis of the anthranilate ATP and was purified by chromatography on Lichroprep RP18 (25-40 μm) using 1 mM triethylammonium acetate as eluent.
후처리
- customwas purified by chromatography on Lichroprep RP18 (25-40 μm)