Gluconic Acid → Gluconic acid hydrazide

Five grams of gluconic acid were suspended in 15 ml of warm MeOH. After the complete dissolution 4 grams of monoBOC-hydrazine were added to the reaction vessel. The solution was refluxed for 36 h. After the addition of monoBOC-hydrazine the suspension disappeared. At the end, the reaction showed a white precipitate which contained most of the product. The solvent was removed under vacuum and the solid product was extracted in a mixture of NaOH sol. 0.1 N/chloroform. The organic phase was removed while the aqueous phase was passed through anionic exchanger resin and lyophilized. Finally, product was dissolved in cold MeOH and deprotected from the BOC-function by bubbling HCl gas into the solution over 30′. The purity of the protected and the deprotected product was checked by TLC, HPLC and the identity was confirmed by ESI-MS.

1. additionwere added to the reaction vessel
2. temperatureThe solution was refluxed for 36 h
3. customThe solvent was removed under vacuum
4. extractionthe solid product was extracted in a mixture of NaOH sol. 0.1 N/chloroform
5. customThe organic phase was removed while the aqueous phase
6. dissolutionFinally, product was dissolved in cold MeOH
7. customby bubbling HCl gas into the solution over 30′

ORD 기록

ord-5a40b4d080624651bfcd4f521e122470

출처 데이터셋

uspto-grants-2009_05

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US07538092B2