N-Acetyl-DL-cysteine → cobalt-dichloride-N-acetyl cysteine

HRID 526286

반응식

HRID 526286 반응식
O.O.O.O.O.O.[Co:7]([Cl:9])[Cl:8].[C:10]([NH:13][C@H:14]([C:17]([OH:19])=[O:18])[CH2:15][SH:16])(=[O:12])[CH3:11].SCC(C(O)=O)NC(C)=O>O>[C:10]([NH:13][C@H:14]([C:17]([OH:19])=[O:18])[CH2:15][SH:16])(=[O:12])[CH3:11].[Co:7]([Cl:9])[Cl:8]

참가 물질

5

반응물 3

HCID 581N-Acetyl-DL-cysteineHCID 12035N-Acetyl-L-CysteineHCID 517166Dichlorocobalt hexahydrate

생성물 1

HCID 59051191cobalt-dichloride-N-acetyl cysteine

시약·촉매·용매 1

HCID 962Water용매

실험 절차

Cobalt chloride hexahydrate (CoCl2-6H2O (mw=237.9 g/mol, available from Acros)) and NAC (N-Acetyl-L-Cysteine, HSCH2CH(NHCOCH3)CO2H (mw=163.19 g/mol, available from Sigma)) was dissolved in water, resulting in the formation of cobalt-dichloride-N-acetyl cysteine (CoCl2—NAC), referred to herein as the Co—NAC contrast agent. All chemicals were purchased from commercial sources and were used without any further purification. For toxicity testing, two different Co—NAC solutions with low (1 wt. %) concentration of cobalt chloride and with high (10%) concentration were prepared. In both cases, the concentration of NAC in the solution was 2 wt. %. The Co—NAC solution with low cobalt chloride concentration was prepared by dissolving 100 mg (0.42 mmol) of CoCl2-6H2O and 200 mg (1.22 mmol) of NAC in 9.7 mL distillated water. The solution with high cobalt chloride concentration was prepared by dissolving 1000 mg (4.2 mmol) of CoCl2-6H2O and 200 mg (1.22 mmol) of NAC in 8.8 mL distilled water. In both cases, the solutions were sonicated at the room temperature for 15 minutes to completely dissolve the components. To avoid any contaminations and bacteria grow, the fresh Co—NAC solutions were prepared every morning for 6 days and immediately delivered for toxicity testing (see, Example 2 below).

출처 및 근거

ORD 기록
ord-d6cd1f62d5454728bb2652b84885f470
출처 데이터셋
uspto-grants-2016_05
특허 번호
US09345790B2

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