Potassium Carbonate → HCID 71812193, 화합물

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(4S,5R)-5-[3,5-Bis(trifluoromethyl)phenyl]-3-{[5-(5-chloro-2-methoxypyridin-3-yl)-2-(3-fluoroazetidin-1-yl)pyrimidin-4-yl]methyl}-4-methyl-1,3-oxazolidin-2-one (EXAMPLE 1, 100 mg, 0.161 mmol), 2,4-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,3-thiazole (38.6 mg, 0.161 mmol) and K2CO3 (66.9 mg, 0.484 mmol) were mixed with 1.5 mL of 4/1 dioxane/water. The mixture was evacuated and charged with nitrogen three times, then {2-[2-(amino-κN)ethyl]phenyl-κC1}(chloro)[di-tert-butyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine]palladium (tert-Butyl X-Phos Precatalyst) (8.3 mg, 0.016 mmol) was added. The reaction was sealed and then heated for 25 minutes at 130° C. in a microwave reactor. The reaction was diluted with 5 mL acetonitrile and filtered through a 1 g RP C18 silica cartridge, eluting with 15 mL acetonitrile until the filtrate was colorless. The filtrate was concentrated prior to RP Prep purification on a X-Bridge 21.2×150 mm RP column. The sample was loaded in 7:3:1 CH3CN/water/DMSO (5 mL), then eluted with 10-100% acetonitrile/water (0.1% NH4OH) linear gradient over 20 minutes. The product of interest eluted in 80% portion of above gradient. Pure fractions (rich cut; impure discarded) were concentrated and lyophilized to give 10 mg product. LCMS (M+H)+: 697.4. 1H NMR (CDCl3, 500 MHz): δ 8.33 (d, J=2.3 Hz, 1H), 8.23 (s, 1H), 7.94 (s, 1H), 7.80 (s, 2H), 7.55 (d, J=2.1 Hz, 1H), 5.71 (d, J=8.5 Hz, 1H), 5.58 (m, 0.5H) 5.47 (m, 0.5H), 4.78 (d, J=17.1 Hz, 1H), 4.54 (m, 2H), 4.37 (m, 3H), 4.06 (d, J=18.4 Hz, 1H), 4.04 (s, 3H), 2.75 (s, 3H), 2.51 (s, 3H), 0.79 (d, J=6.5 Hz, 3H). RTA IC50: 3 nM

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