참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Pyruvate | HCID 107735 | — |
| 반응물 | Demannose | HCID 161658 | — |
| 반응물 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 반응물 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 용매 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 생성물 | 3-Deoxy-L-glycero-L-galacto-2-nonulosonic acid | HCID 86749067 | 78% |
반응 단계
To a 10 mL potassium phosphate buffer (0.1 M, pH 7.5) were added 10 mM DTT, 0.5 M pyruvate, 0.1 M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3−) resin, eluted with zero to 1 M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 특허 번호 | US06168934A |
|---|
| 출처 데이터셋 | uspto-grants-2001_01 |
|---|
| ORD ID | ord-8c7478989ffc47cbb8dbdb7a7938d4f1 |
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| 출처 메모 | uspto-grants-2001_01 |
|---|
