Citric Acid → Thromboxane

HRID 615598노트

반응식

HRID 615598 반응식
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6](O)/[CH:7]=[CH:8]/[C@@H:9]1[C@@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20](O)=O)[C@H:12]2O[O:24][C@@H:10]1[CH2:11]2.C(O)(=O)CC(CC(O)=O)(C(O)=O)O>CCOCC>[CH3:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][C@@H:13]1[C@@H:9]([CH2:8][CH2:7][CH2:6][CH2:5][CH2:4][CH2:3][CH2:2][CH3:1])[O:24][CH2:10][CH2:11][CH2:12]1

참가 물질

4

반응물 2

HCID 311Citric AcidHCID 445049Prostaglandin H2

생성물 1

HCID 114873Thromboxane

시약·촉매·용매 1

HCID 3283Diethyl Ether용매

실험 절차

To a 12×75 mm tube containing [1-14C] PGH2 (2 μg, 0.01 μCi or 0.05 μCi) and inhibitor (1 mM final assay concentration, in 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung mocrosomes (25 μl, containing 400 μg protein) or an aliquot of human platelet microsomes (25 μl, containing ≅130 g protein). The solution was incubated in a 22° water bath for 2 min.; ether (1 ml) and citric acid (25 μMoles) were added, and the tube plunged into an acetone-dry ice bath. The ether extract was transferred to a 1.0 ml Reacti-vial, and the volume reduced under nitrogen to ≅50 μl.

후처리

  1. concentrationassay concentration
  2. additionin 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung mocrosomes (25 μl, containing 400 μg protein)
  3. additionan aliquot of human platelet microsomes (25 μl, containing ≅130 g protein)
  4. customwas incubated in a 22°
  5. customfor 2 min.
  6. customthe tube plunged into an acetone-dry ice bath
  7. extractionThe ether extract

출처 및 근거

ORD 기록
ord-f987717a68094bb3ac24ef7c396c803e
출처 데이터셋
uspto-grants-1985_02
특허 번호
US04500540

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