Dicyclohexylcarbodiimide → Alanine, 3-cyano-N-[(1,1-dimethylethoxy)carbonyl]- (9CI)

HRID 618791

반응식

HRID 618791 반응식
[C:1]([NH:8][C@H:9]([C:14]([OH:16])=[O:15])[CH2:10][C:11](=O)[NH2:12])([O:3][C:4]([CH3:7])([CH3:6])[CH3:5])=[O:2].C1(N=C=NC2CCCCC2)CCCCC1>N1C=CC=CC=1>[C:11]([CH2:10][CH:9]([NH:8][C:1]([O:3][C:4]([CH3:7])([CH3:6])[CH3:5])=[O:2])[C:14]([OH:16])=[O:15])#[N:12]

참가 물질

4

반응물 2

HCID 10868DicyclohexylcarbodiimideHCID 82035N2-((tert-Butoxy)carbonyl)-L-asparagine

생성물 1

HCID 14585624Alanine, 3-cyano-N-[(1,1-dimethylethoxy)carbonyl]- (9CI)수율 95%

시약·촉매·용매 1

HCID 1049Pyridine용매2회

반응 조건

온도
23 °C

실험 절차

20.0 g (86 mmol, 1 equiv.) of Boc-L-asparagine was dissolved in 120 mL of dry pyridine and 20.0 g (97 mmol, 1.3 equiv.) of dicyclohexylcarbodiimide dissolved in 60 mL of dry pyridine was added dropwise over a period of 30 minutes. The reaction was stirred for 3 hours at 23° C. and filtered through a 2 μm nylon filter. The filtrate was concentrated in vacuo on a rotary evaporator and 100 mL of water was added. The pH was adjusted to 10 with 40% NaOH (aq.) and the solution filtered through a 2 μm nylon filter once again. The filtrate was passed through a 120 mL bed of Dowex 50X8-400 ion exchange resin and the resin washed with four column volumes of 1:1 methanol:water. The filtrate was concentrated in vacuo to yield 17.5 g (95% yield) of the title compound as a white solid. 1H-NMR (CD3OD): 4.40 p.p.m (m, 1H); 2.95 p.p.m. (m, 2H); 1.40 p.p.m. (s, 9H).

후처리

  1. additionwas added dropwise over a period of 30 minutes
  2. filtrationfiltered through a 2 μm nylon
  3. filtrationfilter
  4. concentrationThe filtrate was concentrated in vacuo on a rotary evaporator and 100 mL of water
  5. additionwas added
  6. filtrationthe solution filtered through a 2 μm nylon
  7. filtrationfilter once again
  8. washthe resin washed with four column volumes of 1:1 methanol
  9. concentrationThe filtrate was concentrated in vacuo

출처 및 근거

ORD 기록
ord-7c1f9f5f16e84284b5de7705be4dc83a
출처 데이터셋
uspto-grants-1999_09
특허 번호
US05955576

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