D-(+)-Cellobiose (CNP0226604.15) → Epilactose
반응식
[CH2:1]([OH:23])[C@H:2]1[O:7][C@@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].OC1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O>>[CH2:1]([OH:23])[C@H:2]1[O:7][C@@H:6]([O:8][C@@H:9]([C@H:14]([OH:15])[C@H:13]([OH:16])[CH:12]=[O:17])[C@H:10]([OH:11])[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@H:3]1[OH:22]참가 물질
3
실험 절차
Through the specification of the present invention, the activity of the cellobiose 2-epimerase was determined as a lactose 2-epimerase activity, which catalyzes epimerization of lactose to form epilactose, using lactose as the substrate obtainable in highly purified form at low cost. The lactose 2-epimerase activity was measured as follows. To 1,000 μl of substrate solution, which contains lactose to give a final concentration of 35.1 mM and acetate buffer (pH 6.0) to give a final concentration of 20 mM of the reaction solution, 200 μl of a enzyme solution was added to give a 1,200 μl of the reaction solution, followed by reaction at 50° C. for 20 minutes. The reaction was stopped by heating for 10 minutes in a boiling water bath. Then reaction mixture was subjected to HPLC chromatography to determine the amount of epilactose produced by the reaction. The HPLC chromatography was conducted at the following conditions;