Panose → beta-Maltose

HRID 734171

반응식

[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1O.[CH2:35]([OH:68])[C@H:36]1[O:41][C@H:40]([O:42][C@H:43]2[C@H:48]([OH:49])[C@@H:47]([OH:50])[C@@H:46]([O:51][C@H:52]3[C@H:57]([OH:58])[C@@H:56]([OH:59])[C@@H:55]([OH:60])[O:54][C@@H:53]3[CH2:61][OH:62])[O:45][C@@H:44]2[CH2:63][OH:64])[C@H:39]([OH:65])[C@@H:38]([OH:66])[C@@H:37]1[OH:67]>>[CH2:35]([OH:68])[C@H:36]1[O:41][C@H:40]([O:42][C@H:43]2[C@H:48]([OH:49])[C@@H:47]([OH:50])[C@@H:46]([O:51][C@H:52]3[C@H:57]([OH:58])[C@@H:56]([OH:59])[C@@H:55]([O:60][C@H:3]4[C@H:4]([OH:32])[C@@H:5]([OH:31])[C@@H:6]([OH:8])[O:7][C@@H:2]4[CH2:1][OH:34])[O:54][C@@H:53]3[CH2:61][OH:62])[O:45][C@@H:44]2[CH2:63][OH:64])[C@H:39]([OH:65])[C@@H:38]([OH:66])[C@@H:37]1[OH:67].[CH2:9]([OH:8])[C@H:10]1[O:15][C@H:14]([O:16][C@H:17]2[C@H:22]([OH:27])[C@@H:23]([OH:26])[C@H:24]([OH:25])[O:21][C@@H:18]2[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]1[OH:30].[CH2:35]([OH:68])[C@H:36]1[O:41][C@H:40]([O:42][C@H:43]2[C@H:48]([OH:49])[C@@H:47]([OH:50])[C@@H:46]([O:51][C@H:52]3[C@H:57]([OH:58])[C@@H:56]([OH:59])[C@@H:55]([O:30][C@H:11]4[C@H:12]([OH:29])[C@@H:13]([OH:28])[C@@H:14]([O:16][C@H:17]5[C@H:22]([OH:27])[C@@H:23]([OH:26])[C@@H:24]([OH:25])[O:21][C@@H:18]5[CH2:19][OH:20])[O:15][C@@H:10]4[CH2:9][OH:8])[O:54][C@@H:53]3[CH2:61][OH:62])[O:45][C@@H:44]2[CH2:63][OH:64])[C@H:39]([OH:65])[C@@H:38]([OH:66])[C@@H:37]1[OH:67]

참가 물질

반응물 2

HCID 94448Panose

HCID 192826alpha-Maltotriose

생성물 3

HCID 6255beta-Maltose

HCID 446495Alpha-maltotetraose

HCID 13489094alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranose

실험 절차

As evident from the results in Table 23, it was revealed that, after the enzymatic action, glucose and α-isomaltosylglucose alias 62-O-α-glucosylmaltose or panose were mainly formed maltose as a substrate; and maltose and α-isomaltosylglucose alias 63-O-α-glucosylmaltotriose were mainly formed along with small amounts of glucose, maltotetraose, α-isomaltosylglucose alias 62-O-α-glucosylmaltose or panose, and a product X. Also, it was revealed that maltotriose and the product X were mainly formed from maltotetraose as a substrate along with small amounts of maltose, maltopentaose, α-isomaltosylglucose alias 63-O-α-glucosylmaltotriose; and a product Y; and that maltotetraose and the product Y were mainly formed from maltopentaose as a substrate along with small amounts of maltotriose, maltohexaose, and the products X and Z. The product X as a main product from maltotetraose as a substrate and the product Y as a main product from maltopentaose as a substrate were respectively isolated and purified as follows: The products X and Y were respectively purified on HPLC using “YMC PACK ODS-A R355-15S-15 12A”, a separatory HPLC column commercialized by YMC Co., Ltd., Tokyo, Japan, to isolate the product X having a purity of at least 99.9% from the reaction product from maltotetraose in a yield of about 8.3%, d.s.b., and the product Y having a purity of at least 99.9% from the reaction product from maltopentaose in a yield of about 11.5%, d.s.b.

출처 및 근거

ORD 기록: ord-b531abfc4ee8489aaecab5230838cc81
출처 데이터셋: uspto-grants-2009_09
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: US07592160B2

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