Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Glycolic Acid → Formate
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Glycolic Acid | HCID 757 | — |
| 반응물 | alpha-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID | HCID 1221 | — |
| 반응물 | Isobutyric Acid | HCID 6590 | — |
| 반응물 | Sodium Hydroxide | HCID 14798 | — |
| 반응물 | Benzalkonium Chloride | HCID 3014024 | — |
| 반응물 | Hydroxyacetate | HCID 5460308 | — |
| 생성물 | Formate | HCID 283 | — |
| 생성물 | Oxalate | HCID 71081 | — |
| 생성물 | Npc324351 | HCID 3614358 | — |
반응 단계
The reaction in Example 12 was repeated., using a mixture containing glycolic acid (0.500M), AMPA (0.525M), isobutyric acid (0.100M, HPLC internal standard), flavin mononucleotide (0.01 mM), and 0.72 g of Hansenula polymorpha transformant G01 (43.0 IU glycolate oxidase and 39,880 IU catalase) which had been permeabilized by treatment with 0.2% benzalkonium chloride (Lonza Barquat OJ-50) at pH 8.3 (adjusted with 50% NaOH) and 5° C. After 1 h, the HPLC yields of glyoxylate, formate, and oxalate were 50.2%, 47.4%, and 1.1%, respectively, and 2.2% glycolate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 95% and 105%, respectively, of their initial values.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US05559020 |
| 출처 데이터셋 | uspto-grants-1996_09 |
| ORD ID | ord-35d3ab2391ce41708cab3652593ef4d7 |
| 출처 메모 | uspto-grants-1996_09 |