2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid → Fluorescein Methotrexate
반응식
[CH:1]1[CH:2]=[CH:3][C:4]([C:23]([OH:25])=[O:24])=[C:5]([C:7]2[C:17]3[CH:18]=[CH:19][C:20]([OH:22])=[CH:21][C:16]=3[O:15][C:14]3[C:8]=2[CH:9]=[CH:10][C:11]([CH:13]=3)=[O:12])[CH:6]=1.[CH3:26][N:27]([C:41]1[CH:42]=[CH:43][C:44]([C:47]([NH:49][C@H:50]([C:56]([OH:58])=[O:57])[CH2:51][CH2:52][C:53]([OH:55])=[O:54])=[O:48])=[CH:45][CH:46]=1)[CH2:28][C:29]1[CH:30]=[N:31][C:32]2[N:38]=[C:37]([NH2:39])[N:36]=[C:35]([NH2:40])[C:33]=2[N:34]=1>>[CH:1]1[CH:2]=[CH:3][C:4]([C:23]([OH:25])=[O:24])=[C:5]([C:7]2[C:8]3[CH:9]=[CH:10][C:11]([OH:12])=[CH:13][C:14]=3[O:15][C:16]3[C:17]=2[CH:18]=[CH:19][C:20]([CH:21]=3)=[O:22])[CH:6]=1.[CH3:26][N:27]([C:41]1[CH:42]=[CH:43][C:44]([C:47]([NH:49][C@H:50]([C:56]([OH:58])=[O:57])[CH2:51][CH2:52][C:53]([OH:55])=[O:54])=[O:48])=[CH:45][CH:46]=1)[CH2:28][C:29]1[CH:30]=[N:31][C:32]2[N:38]=[C:37]([NH2:39])[N:36]=[C:35]([NH2:40])[C:33]=2[N:34]=1참가 물질
3
실험 절차
2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium containing 10 mM fluorescein-labeled methotrexate (F-MTX, Molecular Probes/Invitrogen). Labeled cells were washed in serum-free culture medium and images were recorded using a fluorescence microscope (Olympus CKX41 microscope equipped with a DP50 digital camera) using a FITC filter set.
후처리
- wait2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium
- washLabeled cells were washed in serum-free culture medium and images
- customequipped with a DP50 digital camera)
- filtration