Phosphate → Glyoxal 2'-deoxyguanosine

HRID 857828

반응식

HRID 857828 반응식
[C@@H:1]1(N2C=CC(=O)NC2=O)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.C(C=O)=O.[NH:21]1[C:30](=[O:31])[C:29]2[NH:28][CH:27]=[N:26][C:25]=2[N:24]=[C:22]1[NH2:23].P([O-])([O-])([O-])=O.B(O)(O)O.C(N(CC(O)=O)CC(O)=O)CN(CC([O-])=O)CC([O-])=O.[Na+].[Na+]>>[CH:3]([CH:5]=[O:8])=[O:4].[C@@H:1]1([N:26]2[C:25]3[N:24]=[C:22]([NH2:23])[NH:21][C:30](=[O:31])[C:29]=3[N:28]=[CH:27]2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1

참가 물질

6

반응물 5

HCID 1061PhosphateHCID 7628Boric AcidHCID 13712Deoxyuridine2회HCID 13020083Ethylenediaminetetraacetic acid disodium saltHCID 136394281Glyoxal guanine2회

생성물 1

HCID 136394279Glyoxal 2'-deoxyguanosine

실험 절차

Glyoxal-2′-deoxyguanosine was synthesized from 2′-deoxyuridine and glyoxal-guanine under the following conditions, by using any one of the aforementioned three sets of enzyme solutions and the Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2. Specifically, 5 mM phosphate buffer (pH 7) containing 30 mM 2′-deoxyuridine, 30 mM glyoxal-guanine, 100 mM boric acid, and 4 mM disodium ethylenediaminetetraacetate was first prepared.

후처리

  1. customthe Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2
  2. customwas first prepared

출처 및 근거

ORD 기록
ord-dc43600221a54b83ac5cb8d4a912c25b
출처 데이터셋
uspto-grants-2006_12
특허 번호
US07148042B2

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