Ethanol → Ethinylestradiol

HRID 879905노트

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HRID 879905 반응식
[CH3:1][C@@:2]12[C@@H:18]([OH:19])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2.[CH2:21](O)[CH3:22]>P([O-])([O-])([O-])=O>[CH3:1][C@@:2]12[C@:18]([OH:19])([C:21]#[CH:22])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2

참가 물질

4

반응물 2

HCID 702EthanolHCID 5757Estradiol

생성물 1

HCID 5991Ethinylestradiol

시약·촉매·용매 1

HCID 1061Phosphate용매

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온도
37 °C

실험 절차

Estradiol conjugate (33.5 mg, 0.039 mmol), prepared from Conjugate 1 as described in Example 16, was dissolved in 58 mL of a 9:4 ethanol/0.05 M aqueous phosphate buffer (pH 7.4) mixture, and the solution was shaken in a 37° C. incubator for 9 days. Six 8 mL aliquots were collected at 1-, 2-, 3-, 5-, 7-, and 9-day intervals. Estradiol release was quantified by adding 33 μL of a 0.09 M solution of butylated hydroxytoluene (BHT) in deuterochloroform as an internal standard, then comparing integrals of 1H NMR signals corresponding to the steroid and the internal standard. Results are shown in Table 4 below.

후처리

  1. customSix 8 mL aliquots were collected at 1-, 2-, 3-, 5-, 7-, and 9-day intervals
  2. additionby adding 33 μL of a 0.09 M solution of butylated hydroxytoluene (BHT) in deuterochloroform as an internal standard
  3. customResults

출처 및 근거

ORD 기록
ord-7a8a9a94b78c43e189ad97367162b27d
출처 데이터셋
uspto-grants-2015_03
특허 번호
US08992977B2

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