Ampicillin → (+)-Pantolactone

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A full inoculating loop of E. coli XL1Blue pKS+681 was grown (overnight, 37° C.) on an LB plate with ampicillin (100 μg/ml), IPTG (0.2 mM) and X-Gal (80 mg/l) and then resuspended (OD600=2.5) in 0.5 ml of Tris-HCl pH 7.0 and 50 mM D,L-pantolactone. A corresponding E. coli XL1Blue pBluescriptKS+-sample (OD600=2.5) was a useful comparison. After 1 h, the cells were centrifuged down and D,L-pantolactone, D,L-, D-, and L-pantoic acid were determined by HPLC analysis. Tab. 6a shows the activities and ee values for the various samples. The suspension had an activity of >90 U/L. However, no significant activity was evident in liquid culture batches (cf. Example 12).

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