반응 단계
A solution of isopropylmagnesium chloride-lithium chloride complex in tetrahydrofuran (1.3 M, 0.73 mL, 0.94 mmol) was added dropwise to an ice-water cooled, stirring solution of 3-(4-(1H-1,2,4-triazol-1-yl)benzyl)-4-chloro-6-iodo-2-methoxyquinoline (300 mg, 0.63 mmol, Intermediate 20: step c) in dry tetrahydrofuran (6 mL). After 10 minutes, a solution of 1-(4-benzoylpiperidin-1-yl)ethanone (218 mg, 0.94 mmol, Intermediate 18) in tetrahydrofuran (5 mL) was added dropwise. After 30 minutes, the flask was warmed to 23° C. After 1 hour, the flask was warmed to 55° C. After 30 minutes, the flask was allowed to cool to 23° C. Water (20 mL), saturated aqueous sodium chloride solution (20 mL), and ethyl acetate (100 mL) were added. The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Silica gel (5 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash column chromatography purification. Elution with dichloromethane initially, grading to 5% methanol-dichloromethane provided the title compound which was further purified by RP-HPLC eluting initially with 5% acetonitrile-water (containing 0.05% trifluoroacetic acid), grading to 95% acetonitrile-water (containing 0.05% trifluoroacetic acid) to provide the title compound as a white solid after partitioning the purified material between dichloromethane-saturated aqueous sodium bicarbonate solution, separating the layers, drying the organic layer with sodium sulfate, filtering the dried solution, and concentrating the filtrate to dryness. 1H NMR (500 MHz, CDCl3) δ ppm 8.47 (s, 1H), 8.32-8.25 (m, 1H), 8.06 (s, 1H), 7.80-7.72 (m, 1H), 7.72-7.62 (m, 1H), 7.57-7.48 (m, 4H), 7.44-7.38 (m, 2H), 7.37-7.29 (m, 2H), 7.26-7.18 (m, 1H), 4.75-4.63 (m, 1H), 4.34 (s, 2H), 4.05 (s, 3H), 3.90-3.75 (m, 1H), 3.17-2.99 (m, 1H), 2.76 (t, J=11.8 Hz, 1H), 2.66-2.49 (m, 1H), 2.28 (s, 1H, rotamer), 2.27 (s, 1H, rotamer), 2.05 (s, 3H, rotamer), 2.04 (s, 3H, rotamer), 1.75-1.62 (m, 1H), 1.55-1.28 (m, 3H); MS (ESI): mass calcd. for C33H32ClN5O3, 581.2. m/z found, 582.0 [M+H]+.
