CC1C(=C)C(C(C(C(NC)CC2C=CC=CC=2)=O)C)CC(=O)NCCCC(C(C)=O)CC(=C)C(CC2N=CNC=2)C(C)C(=O)C(CC2C=CC=CC=2)CC(=[O:5])C1CCCCN.[C:62]([O-:65])(=[O:64])[CH3:63].[N+:66]([C:69]1[CH:74]=[CH:73][C:72](CC([O-])=O)=[CH:71][CH:70]=1)([O-:68])=[O:67]>CN(C)C=O>[C:62]([O:65][C:72]1[CH:73]=[CH:74][C:69]([N+:66]([O-:68])=[O:67])=[CH:70][CH:71]=1)(=[O:64])[CH3:63].[N+:66]([C:69]1[CH:74]=[CH:73][C:72]([OH:5])=[CH:71][CH:70]=1)([O-:68])=[O:67]A solution of 10 mg of calcitonine M acetate in 2 ml of absolute dimethylformamide is treated with 0.5 ml of a 1% solution of para-nitrophenylacetate in dimethylformamide, rinsed with nitrogen, and allowed to stand in the closed test tube at 40° C. for 30 minutes. 5 ml of 2N-acetic acid are then added, the excess para-nitrophenyl acetate and the para-nitrophenol formed are extracted with ethyl acetate (2 × 30 ml), the aqueous phase is evaporated to dryness under reduced pressure, the residue dissolved in 0.5 ml of 95% acetic acid, and the solution lyophilized. The product consists of a mixture of mono- and diacetyl derivative and some starting material. The monoacetyl derivative itself is a mixture of the Nα - and Nε -derivatives as evidenced by electrophoresis at pH 8.
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